{"id":6249,"date":"2026-10-19T09:48:41","date_gmt":"2026-10-19T09:48:41","guid":{"rendered":"https:\/\/phd.hi.is\/doktorsvorn\/doktorsvorn-i-lifverkfraedi-thordis-kristjansdottir\/"},"modified":"2026-10-19T09:48:41","modified_gmt":"2026-10-19T09:48:41","slug":"doktorsvorn-i-lifverkfraedi-thordis-kristjansdottir","status":"publish","type":"phd_defence","link":"https:\/\/phd.hi.is\/en\/doktorsvorn\/doktorsvorn-i-lifverkfraedi-thordis-kristjansdottir\/","title":{"rendered":"Doktorsv\u00f6rn \u00ed l\u00edfverkfr\u00e6\u00f0i- \u00de\u00f3rd\u00eds Kristj\u00e1nsd\u00f3ttir"},"content":{"rendered":"<div class=\"field-items\">\n<div class=\"field-item even\" property=\"content:encoded\">\n<p><a href=\"https:\/\/livestream.com\/hi\/doktorsvornthordiskrisjansdottir\">V\u00f6rninni ver\u00f0ur streymt<\/a><\/p>\n<p><strong>Doktorsefni:<\/strong>\u00a0\u00de\u00f3rd\u00eds Kristj\u00e1nsd\u00f3ttir<\/p>\n<p><strong>Heiti ritger\u00f0ar:<\/strong>\u00a0\u00der\u00f3un l\u00edtt rannsaka\u00f0ra bakter\u00eda sem frumuverksmi\u00f0jur: \u00c1hersla \u00e1 efnaskiptal\u00edk\u00f6n og -verkfr\u00e6\u00f0i fyrir Rhodothermus marinus og Lactobacillus reuteri (Development of non-model bacteria as cell factories: Focusing on metabolic modelling and engineering of Rhodothermus marinus and Lactobacillus reuteri)\u00a0<\/p>\n<p><strong>Andm\u00e6lendur: <br \/> <\/strong>Dr. Sheila Ingemann Jensen, s\u00e9rfr\u00e6\u00f0ingur vi\u00f0 DTU, T\u00e6knih\u00e1sk\u00f3lann \u00ed Danm\u00f6rku<br \/> Dr. Eduard Kerkhoven, d\u00f3sent vi\u00f0 Chalmers t\u00e6knih\u00e1sk\u00f3lann \u00ed Gautaborg, Sv\u00ed\u00fej\u00f3\u00f0<\/p>\n<p><strong>Lei\u00f0beinendur:<br \/> <\/strong>Dr.\u00a0Steinn Gu\u00f0mundsson, pr\u00f3fessor vi\u00f0 I\u00f0na\u00f0arverkfr\u00e6\u00f0i-, v\u00e9laverkfr\u00e6\u00f0i- og t\u00f6lvunarfr\u00e6\u00f0ideild H\u00e1sk\u00f3la \u00cdslands<br \/> Dr. Gu\u00f0mundur \u00d3li Hreggvi\u00f0sson, pr\u00f3fessor vi\u00f0 L\u00edf- og umhverfisv\u00edsindadeild H\u00e1sk\u00f3la \u00cdslands\u00a0<\/p>\n<p><strong>Einnig \u00ed doktorsnefnd:<\/strong><strong><br \/> <\/strong>Dr. \u00d3lafur H. Fri\u00f0j\u00f3nsson, fagstj\u00f3ri hj\u00e1 Mat\u00eds<br \/> Dr. Markus J. Herrg\u00e5rd, s\u00e9rfr\u00e6\u00f0ingur vi\u00f0 BioInnovation Institute, Danm\u00f6rku\u00a0<\/p>\n<p><strong>Doktorsv\u00f6rn st\u00fdrir:<\/strong>\u00a0Dr. R\u00fanar Unn\u00fe\u00f3rsson, pr\u00f3fessor og deildarforseti I\u00f0na\u00f0arverkfr\u00e6\u00f0i-, v\u00e9laverkfr\u00e6\u00f0i- og t\u00f6lvunarfr\u00e6\u00f0ideildar H\u00e1sk\u00f3la \u00cdslands\u00a0<\/p>\n<p>Verkefni\u00f0 var unni\u00f0 \u00ed samstarfi milli I\u00f0na\u00f0arverkfr\u00e6\u00f0i-, v\u00e9laverkfr\u00e6\u00f0i- og t\u00f6lvunarfr\u00e6\u00f0ideildar, L\u00edf- og umhverfisv\u00edsindadeildar og Mat\u00eds.<\/p>\n<p><strong>\u00c1grip<br \/><\/strong>Vegna loftslagsbreytinga og offj\u00f6lgunar mannkyns er mikil \u00fe\u00f6rf \u00e1 sj\u00e1lfb\u00e6rum a\u00f0fer\u00f0um \u00ed efnai\u00f0na\u00f0i, s.s. notkun l\u00edfmassavera. Markmi\u00f0 \u00feessa verkefnis var a\u00f0 rannsaka m\u00f6guleikann \u00e1 a\u00f0 nota tv\u00e6r \u00f6rverur, sem ekki eru skilgreindar sem tilraunal\u00edfverur, \u00fe.e. eru tilt\u00f6lulega l\u00edtt \u00feekktar, til framlei\u00f0slu i\u00f0na\u00f0arefna \u00far endurn\u00fdjanlegum l\u00edfmassa. Til \u00feess voru efnaskiptal\u00edk\u00f6n og -verkfr\u00e6\u00f0i notu\u00f0. Yfirleitt eru tilraunal\u00edfverur nota\u00f0ar sem frumuverksmi\u00f0jur, en \u00fea\u00f0 getur veri\u00f0 \u00f3\u00e1kj\u00f3sanlegt a\u00f0 nota \u00fe\u00e6r \u00ed l\u00edfmassaverum \u00fear sem umhverfi\u00f0 getur veri\u00f0 \u00f6fgakennt.\u00a0Rhodothermus marinus\u00a0er hitak\u00e6r bakter\u00eda sem getur nota\u00f0 fj\u00f6lsykrur \u00far fl\u00f3knum l\u00edfmassa og framleitt kar\u00f3ten\u00f3\u00ed\u00f0a.\u00a0Lactobacillus reuteri\u00a0er bakter\u00eda sem getur vaxi\u00f0 vi\u00f0 breytt hitastigsbil mi\u00f0a\u00f0 vi\u00f0 a\u00f0rar LAB og getur b\u00fai\u00f0 n\u00e1tt\u00farulega til 1,3-pr\u00f3paned\u00ed\u00f3l og B12 v\u00edtam\u00edn.<br \/>Efnaskiptal\u00edk\u00f6n af b\u00e1\u00f0um \u00feessum bakter\u00edum voru sm\u00ed\u00f0u\u00f0, fari\u00f0 var yfir \u00feau handvirkt og \u00feau pr\u00f3fu\u00f0.\u00a0L. reuteri\u00a0l\u00edkani\u00f0 var nota\u00f0 til a\u00f0 sp\u00e1 fyrir um hvernig \u00fatsl\u00e1ttur \u00e1kve\u00f0ins gens og mismunandi kolefnisgjafar haf\u00f0i \u00e1hrif \u00e1 fl\u00e6\u00f0i helstu l\u00edfefna \u00ed mi\u00f0l\u00e6gu efnaskiptanetinu og til a\u00f0 finna lei\u00f0ir til a\u00f0 b\u00faa til 1-pr\u00f3pan\u00f3l. \u00de\u00f3 nokkrir hlutar af efnaskiptaneti\u00a0R. marinus\u00a0voru sko\u00f0a\u00f0ir s\u00e9rstaklega og \u00ed kj\u00f6lfari\u00f0 var frumu\u00fe\u00e9ttni og kar\u00f3ten\u00f3\u00ed\u00f0framlei\u00f0sla ranns\u00f6ku\u00f0 vi\u00f0 mismunandi vaxtar- og arfger\u00f0ara\u00f0st\u00e6\u00f0ur.<br \/>R. marinus\u00a0framlei\u00f0ir n\u00e1tt\u00farulega \u03b3-kar\u00f3ten\u00f3\u00ed\u00f0a \u00ed miklu magni. Kar\u00f3ten\u00f3\u00ed\u00f0 n\u00fdmyndunargenin og -ferillinn \u00ed\u00a0R. marinus\u00a0voru ranns\u00f6ku\u00f0 me\u00f0 \u00fea\u00f0 a\u00f0 markmi\u00f0i a\u00f0 \u00fatb\u00faa grunnstofn fyrir framlei\u00f0slu \u00e1 i\u00f0na\u00f0arkar\u00f3ten\u00f3\u00ed\u00f0um. \u00cd framhaldinu f\u00e9kkst st\u00f6kkbrig\u00f0i sem b\u00fdr til lycopene, sem er sameiginlegur forveri margra kar\u00f3ten\u00f3\u00ed\u00f0a. \u00deetta er fyrsti \u00feekkti\u00a0R. marinus\u00a0stofninn sem b\u00fdr til marka\u00f0sv\u00e6nt kar\u00f3ten\u00f3\u00ed\u00f0, og \u00a0\u00fev\u00ed er \u00fea\u00f0 mikilv\u00e6gt skref \u00ed \u00e1tt a\u00f0 stofni sem h\u00e6gt er a\u00f0 nota \u00ed i\u00f0na\u00f0i.<br \/>N\u00faverandi a\u00f0fer\u00f0ir sem nota\u00f0ar eru til a\u00f0 erf\u00f0abreyta\u00a0R. marinus\u00a0eru takmarka\u00f0ar af fj\u00f6lda valgena, sem erfitt er a\u00f0 endurnota fyrir frekari erf\u00f0abreytingar. Hita\u00feoli\u00f0 CRISPR-Cas9 kerfi var \u00fer\u00f3a\u00f0, sem gefur vonir um v\u00ed\u00f0t\u00e6kari og au\u00f0veldari erf\u00f0abreytingar \u00ed\u00a0R. marinus\u00a0\u00ed framt\u00ed\u00f0inni. Afrakstur verkefnisins er aukinn skilningur \u00e1 efnaskiptum\u00a0R. marinus\u00a0og\u00a0L. reuteri.\u00a0Efnaskiptal\u00edk\u00f6nin sem sm\u00ed\u00f0u\u00f0 voru auka m\u00f6guleika \u00e1 hagn\u00fdtingu og\u00a0R. marinus\u00a0st\u00f6kkbrig\u00f0i\u00f0 sem framlei\u00f0ir lycopene er h\u00e6gt a\u00f0 nota sem grunnstofn fyrir frekari st\u00f6kkbreytingar til framlei\u00f0slu fleiri i\u00f0na\u00f0arkar\u00f3ten\u00f3\u00ed\u00f0a.<\/p>\n<p><strong>Um doktorsefni\u00f0<\/strong><\/p>\n<p>\u00de\u00f3rd\u00eds Kristj\u00e1nsd\u00f3ttir er f\u00e6dd 10. j\u00fal\u00ed 1989 og uppalin \u00ed Reykjav\u00edk. H\u00fan lauk st\u00fadentspr\u00f3fi fr\u00e1 Verzlunarsk\u00f3la \u00cdslands 2009, BS-pr\u00f3fi \u00ed l\u00edfefnafr\u00e6\u00f0i fr\u00e1 H\u00e1sk\u00f3la \u00cdslands 2013 og MS-pr\u00f3fi \u00ed kerfisl\u00edffr\u00e6\u00f0i fr\u00e1 DTU, T\u00e6knih\u00e1sk\u00f3lanum \u00ed Danm\u00f6rku, 2015. H\u00fan h\u00f3f st\u00f6rf hj\u00e1 Mat\u00eds a\u00f0 loknu meistaran\u00e1mi og byrja\u00f0i \u00ed doktorsn\u00e1mi 2016 sem h\u00fan hefur stunda\u00f0 me\u00f0fram \u00f6\u00f0rum verkefnum hj\u00e1 Mat\u00eds. Foreldrar \u00de\u00f3rd\u00edsar eru dr. Sigr\u00ed\u00f0ur Hj\u00f6rleifsd\u00f3ttir l\u00edffr\u00e6\u00f0ingur og Kristj\u00e1n G. Sveinsson verkfr\u00e6\u00f0ingur. \u00de\u00f3rd\u00eds er gift Bjarka \u00d3marssyni verkfr\u00e6\u00f0ingi.<\/p>\n<p><strong><br \/> <\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<\/div><\/div>","protected":false},"featured_media":5068,"template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_phd_date_start":"2022-02-01T13:00:00+00:00","_phd_date_end":"2022-02-01T15:00:00+00:00","_phd_location":"A\u00f0albygging H\u00e1t\u00ed\u00f0arsalur","_phd_admission":"\u00d6ll velkomin","_phd_thesis_title":"\u00der\u00f3un l\u00edtt rannsaka\u00f0ra bakter\u00eda sem frumuverksmi\u00f0jur: \u00c1hersla \u00e1 efnaskiptal\u00edk\u00f6n og -verkfr\u00e6\u00f0i fyrir Rhodothermus marinus og Lactobacillus reuteri (Development of non-model bacteria as cell factories: Focusing on metabolic modelling and engineering of Rhodothermus marinus and Lactobacillus reuteri)","_phd_source_url":"https:\/\/gamli.hi.is\/vidburdir\/doktorsvorn_i_lifverkfraedi_thordis_kristjansdottir","_phd_source_node_id":"381817","_phd_source_type":"vidburdir","_phd_member_id":776,"footnotes":""},"phd_school":[108,93],"phd_discipline":[129],"class_list":["post-6249","phd_defence","type-phd_defence","status-publish","has-post-thumbnail","hentry","phd_school-idnadarverkfraedi-velaverkfraedi-og-tolvunarfraedideild","phd_school-verkfraedi-og-natturuvisindasvid","phd_discipline-phd-i-lifverkfraedi"],"_links":{"self":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence\/6249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence"}],"about":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/types\/phd_defence"}],"version-history":[{"count":0,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence\/6249\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/media\/5068"}],"wp:attachment":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/media?parent=6249"}],"wp:term":[{"taxonomy":"phd_school","embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_school?post=6249"},{"taxonomy":"phd_discipline","embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_discipline?post=6249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}