{"id":6226,"date":"2026-10-19T09:48:39","date_gmt":"2026-10-19T09:48:39","guid":{"rendered":"https:\/\/phd.hi.is\/doktorsvorn\/doktorsvorn-i-efnafraedi-mostafa-ghasemisarabbadieh\/"},"modified":"2026-10-19T09:48:39","modified_gmt":"2026-10-19T09:48:39","slug":"doktorsvorn-i-efnafraedi-mostafa-ghasemisarabbadieh","status":"publish","type":"phd_defence","link":"https:\/\/phd.hi.is\/en\/doktorsvorn\/doktorsvorn-i-efnafraedi-mostafa-ghasemisarabbadieh\/","title":{"rendered":"Doktorsv\u00f6rn \u00ed efnafr\u00e6\u00f0i – Mostafa Ghasemisarabbadieh"},"content":{"rendered":"
\n
\n

Streymi\u00a0<\/strong><\/a><\/p>\n

Doktorsefni:<\/strong>\u00a0Mostafa Ghasemisarabbadieh<\/p>\n

Heiti ritger\u00f0ar:<\/strong>\u00a0Hitast\u00f6\u00f0gun ox\u00fdt\u00f3sins og fibroblast vaxtar\u00fe\u00e1ttar 2 (Thermal stabilization of oxytocin and fibroblast growth factor 2)<\/p>\n

Andm\u00e6lendur:
<\/strong>Dr. Kazimierz Wisniewski, forst\u00f6\u00f0uma\u00f0ur vi\u00f0 Peptide Logic LLC \u00ed San Diego, Bandar\u00edkjunum
Dr. Felix Khuluza, d\u00f3sent vi\u00f0 Kamuzu University of Health Sciences \u00ed Blantyre, Malawi<\/p>\n

Lei\u00f0beinendur: <\/strong>Dr.\u00a0Benjam\u00edn Ragnar Sveinbj\u00f6rnsson, lektor vi\u00f0 Raunv\u00edsindadeild H\u00e1sk\u00f3la \u00cdslands, og dr. Sveinbj\u00f6rn Gizurarson, pr\u00f3fessor vi\u00f0 Lyfjafr\u00e6\u00f0ideild H\u00e1sk\u00f3la \u00cdslands<\/p>\n

Einnig \u00ed doktorsnefnd<\/strong>:
<\/strong>Dr. Au\u00f0ur \u00d6nnu Magn\u00fasd\u00f3ttir, framkv\u00e6mdastj\u00f3ri Landverndar
Dr. P\u00e9tur Orri Hei\u00f0arsson, d\u00f3sent vi\u00f0 Raunv\u00edsindadeild H\u00e1sk\u00f3la \u00cdslands<\/p>\n

Doktorsv\u00f6rn st\u00fdrir:<\/strong>\u00a0Dr. Einar \u00d6rn Sveinbj\u00f6rnsson, pr\u00f3fessor og deildarforseti Raunv\u00edsindadeildar H\u00e1sk\u00f3la \u00cdslands<\/p>\n

\u00c1grip<\/strong><\/p>\n

Ox\u00fdt\u00f3sin er leg\u00e6\u00f0a taugapept\u00ed\u00f0 sem Al\u00fej\u00f3\u00f0aheilbrig\u00f0ism\u00e1lastofnunin r\u00e1\u00f0leggur a\u00f0 s\u00e9 nota\u00f0 sem fyrsta stigs v\u00f6rn til a\u00f0 fyrirbyggja og me\u00f0h\u00f6ndla bl\u00e6\u00f0ingar eftir f\u00e6\u00f0ingu. Bl\u00e6\u00f0ingar eftir f\u00e6\u00f0ingu eru a\u00f0ald\u00e1narors\u00f6k m\u00e6\u00f0ra (27,1%) \u00ed m\u00f6rgum l\u00f6ndum \u00fear sem tekjur eru l\u00e6gri. Bas\u00edski fibroblast vaxtar\u00fe\u00e1tturinn (FGF2) er hluti af fj\u00f6lskyldu fibroblast vaxta\u00fe\u00e1tta. \u00dea\u00f0 tekur \u00fe\u00e1tt \u00ed a\u00f0 st\u00fdra \u00fdmissi frumustarfsemi, \u00fear \u00e1 me\u00f0al frumufj\u00f6lgun, fl\u00e6\u00f0i, a\u00f0greiningu, sem og \u00e6\u00f0amyndun \u00ed \u00fdmsum vefjum svo sem h\u00fa\u00f0, \u00e6\u00f0um, v\u00f6\u00f0vum, fitu, sinum\/li\u00f0b\u00f6ndum, brj\u00f3ski, beinum, t\u00f6nnum og taugum.<\/p>\n

B\u00e6\u00f0i FGF2 og ox\u00fdt\u00f3sin eru \u00f3st\u00f6\u00f0ug og brotna hratt ni\u00f0ur vi\u00f0 stofuhita. S\u00fdnt hefur veri\u00f0 fram \u00e1 a\u00f0 FGF2 vatnslausnir eru einungis st\u00f6\u00f0ugar \u00ed um viku vi\u00f0 4\u00b0C og g\u00f6gn hafa s\u00fdnt a\u00f0 ox\u00fdt\u00f3sin \u00feolir ekki geymsu vi\u00f0 30\u00b0C \u00ed meira en m\u00e1nu\u00f0 e\u00f0a 2 vikur vi\u00f0 40\u00b0C. Markmi\u00f0 \u00feessa verkefnis var a\u00f0 auka hitast\u00f6\u00f0ugleika ox\u00fdt\u00f3sins og gera frumranns\u00f3knir \u00e1 FGF2 \u00ed vatnslausnum til a\u00f0 auka geymslu\u00feol \u00feessara efna.<\/p>\n

D-(+)-gl\u00fak\u00f3sam\u00edn-h\u00fddr\u00f3kl\u00f3r\u00ed\u00f0, tetraet\u00fdlengl\u00fdk\u00f3l (4EG), N-acet\u00fdl-D-gl\u00fak\u00f3sam\u00edn og blanda af \u00feessum vi\u00f0b\u00f3tarefnum voru pr\u00f3fu\u00f0 til a\u00f0 sj\u00e1 \u00e1hrif \u00feeirra \u00e1 st\u00f6\u00f0ugleika ox\u00fdt\u00f3sins og FGF2. Ni\u00f0urst\u00f6\u00f0ur ranns\u00f3knarinnar s\u00fdna a\u00f0 \u00f6ll \u00feessi efni ger\u00f0u FGF2 \u00f3st\u00f6\u00f0ugra \u00ed fosfat-buffer sal\u00edn (PBS) lausn. 4EG og D-(+) gl\u00fak\u00f3sam\u00edn-h\u00fddrokl\u00f3r\u00ed\u00f0 ger\u00f0u ox\u00fdt\u00f3sin \u00f3st\u00f6\u00f0ugra \u00ed b\u00e6\u00f0i fosfat og asetat stu\u00f0p\u00fa\u00f0alausnum. N-acet\u00fdl-D-gl\u00fak\u00f3sam\u00edn s\u00fdndi hverfandi e\u00f0a hugsanlega \u00f6rl\u00edti\u00f0 st\u00f6\u00f0gandi \u00e1hrif \u00e1 ox\u00fdt\u00f3sin.<\/p>\n

\u00c1hrif trehal\u00f3sa \u00e1 st\u00f6\u00f0ugleika FGF2 og ox\u00fdt\u00f3sins \u00ed vatns-stu\u00f0p\u00fa\u00f0alausnum var l\u00edka pr\u00f3fa\u00f0. \u00der\u00e1tt fyrir a\u00f0 trehal\u00f3si s\u00fdndi l\u00edtil e\u00f0a hverfandi \u00e1hrif \u00e1 st\u00f6\u00f0ugleika ox\u00fdt\u00f3sins jafnvel vi\u00f0 allt a\u00f0 1,0 M styrk \u00ed asetat-stu\u00f0p\u00fa\u00f0alausn, \u00fe\u00e1 h\u00e6g\u00f0i \u00fea\u00f0 \u00e1 ni\u00f0urbrotshra\u00f0a FGF2 \u00ed vi\u00f0urvist allar stu\u00f0p\u00fa\u00f0alausnanna sem voru\u00f0 nota\u00f0ir (HEPES, TRIS, s\u00edtrate\/fosfat og PBS).<\/p>\n

\u00c1hrif mismunandi stu\u00f0p\u00fa\u00f0alausna voru l\u00edka metin. Ox\u00fdt\u00f3sin reyndist vera st\u00f6\u00f0ugra \u00ed acetat-stu\u00f0p\u00fa\u00f0alausn heldur en s\u00edtrat\/fosfat e\u00f0a fosfat stu\u00f0p\u00fa\u00f0alausn og vi\u00f0 s\u00e1um a\u00f0 st\u00f6\u00f0ugleikinn er l\u00edka stykh\u00e1\u00f0ur, \u00fear sem asetat stu\u00f0p\u00fa\u00f0alausnir me\u00f0 styrk 0,025 M e\u00f0a l\u00e6gra er betra. FGF2 var st\u00f6\u00f0ugast \u00ed PBS \u00ed samanbur\u00f0i vi\u00f0 a\u00f0ra stu\u00f0p\u00fa\u00f0a sem voru nota\u00f0ir.<\/p>\n

\u00c1hrif k\u00f3r\u00f3nuetera \u00e1 st\u00f6\u00f0ugleika ox\u00fdt\u00f3sins og FGF2 \u00ed vatnslausnum var l\u00edka kanna\u00f0. 18-k\u00f3r\u00f3na-6 og 12-k\u00f3r\u00f3na-4 h\u00f6f\u00f0u neikv\u00e6\u00f0 \u00e1hrif \u00e1 st\u00f6\u00f0ugleika FGF2. Vi\u00f0 s\u00e1um a\u00f0 \u00e1 me\u00f0an 12-k\u00f3r\u00f3na-4 og 15-k\u00f3r\u00f3na-5 h\u00f6f\u00f0u ekki st\u00f6\u00f0gandi \u00e1hrif \u00e1 ox\u00fdt\u00f3sin, \u00fe\u00e1 haf\u00f0i 18-k\u00f3r\u00f3na-6 st\u00f6\u00f0gandi \u00e1hrif \u00e1 \u00f3x\u00fdt\u00f3sin \u00ed s\u00edtrate\/fosfat stu\u00f0p\u00fa\u00f0alausn vi\u00f0 pH 4,5. Hins vegar haf\u00f0i vi\u00f0urvist 18-k\u00f3r\u00f3na-6 neikv\u00e6\u00f0 \u00e1hrif \u00e1 st\u00f6\u00f0ugleikann \u00ed asetat-stu\u00f0p\u00fa\u00f0alausn vi\u00f0 sama pH, og leiddi m\u00f6gulega til annars ni\u00f0urbrotsferlis.<\/p>\n

\u00c1hrif andoxunarefna, svo sem \u00fevags\u00fdru, b\u00fat\u00fdla\u00f0-h\u00fddrox\u00fdt\u00f3luens (BHT) og L-ascorbic s\u00fdru var l\u00edka pr\u00f3fa\u00f0 fyrir st\u00f6\u00f0ugleika ox\u00fdt\u00f3sins \u00ed lausn. \u00der\u00e1tt fyrir a\u00f0 \u00feekktar ni\u00f0urbrotslei\u00f0ir fyrir ox\u00fdt\u00f3sin innihaldi oxun \u00e1 \u00e1kve\u00f0num am\u00edn\u00f3s\u00fdrum, h\u00f6f\u00f0u andoxunarefnin \u00fevags\u00fdra og BHT hverfandi \u00e1hrif \u00e1 st\u00f6\u00f0ugleika ox\u00fdt\u00f3sins \u00e1 me\u00f0an L-ascorbic s\u00fdran leiddi til hra\u00f0ara ni\u00f0urbrots.<\/p>\n

\u00c1hrif mismunandi s\u00fdrustiga \u00e1 st\u00f6\u00f0ugleika FGF2 \u00ed s\u00edtrat\/fosfat stu\u00f0p\u00fa\u00f0alausn vi\u00f0 pH 6-8 og asetat stu\u00f0p\u00fa\u00f0alausn vi\u00f0 pH 4,5 og 5,5 s\u00fdndi a\u00f0 l\u00edkt og \u00f6nnur pr\u00f3t\u00edn \u00ed FGF fj\u00f6lskyldunni, \u00fe\u00e1 er FGF2 st\u00f6\u00f0ugra vi\u00f0 s\u00fdrustig sem er n\u00e1l\u00e6gt hlutlausu e\u00f0a \u00f6rl\u00edti\u00f0 bas\u00edskt \u00ed samanbur\u00f0i vi\u00f0 s\u00fart pH.<\/p>\n

Um doktorsefni\u00f0<\/strong><\/p>\n

Mostafa er fr\u00e1 \u00cdran, f\u00e6ddur 1984 og uppalinn \u00ed borginni Kermanshah. Hann lauk B.Sc. \u00ed hagn\u00fdttri efnafr\u00e6\u00f0i fr\u00e1 Payam Noor h\u00e1sk\u00f3lanum og M.Sc. \u00ed l\u00edfr\u00e6nni efnafr\u00e6\u00f0i fr\u00e1 Imam Hossein h\u00e1sk\u00f3lanum \u00ed Teheran \u00ed \u00cdran. Hann h\u00f3f doktorsn\u00e1mi\u00f0 vi\u00f0 H\u00e1sk\u00f3la \u00cdslands \u00e1ri\u00f0 2018 og \u00fea\u00f0 er honum sannur hei\u00f0ur a\u00f0 verja n\u00fa doktorsritger\u00f0 s\u00edna h\u00e9r.<\/p>\n<\/div><\/div>","protected":false},"featured_media":4991,"template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_phd_date_start":"2021-09-22T14:00:00+00:00","_phd_date_end":"2021-09-22T16:00:00+00:00","_phd_location":"A\u00f0albygging H\u00e1t\u00ed\u00f0arsalur","_phd_admission":"A\u00f0gangur \u00f3keypis","_phd_thesis_title":"Hitast\u00f6\u00f0gun ox\u00fdt\u00f3sins og fibroblast vaxtar\u00fe\u00e1ttar 2 (Thermal stabilization of oxytocin and fibroblast growth factor 2)","_phd_source_url":"https:\/\/gamli.hi.is\/vidburdir\/doktorsvorn_i_efnafraedi_mostafa_ghasemisarabbadieh","_phd_source_node_id":"378633","_phd_source_type":"vidburdir","_phd_member_id":750,"footnotes":""},"phd_school":[101,93],"phd_discipline":[102],"class_list":["post-6226","phd_defence","type-phd_defence","status-publish","has-post-thumbnail","hentry","phd_school-raunvisindadeild","phd_school-verkfraedi-og-natturuvisindasvid","phd_discipline-phd-i-efnafraedi"],"_links":{"self":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence\/6226","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence"}],"about":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/types\/phd_defence"}],"version-history":[{"count":0,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence\/6226\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/media\/4991"}],"wp:attachment":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/media?parent=6226"}],"wp:term":[{"taxonomy":"phd_school","embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_school?post=6226"},{"taxonomy":"phd_discipline","embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_discipline?post=6226"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}