{"id":6202,"date":"2026-10-19T09:48:37","date_gmt":"2026-10-19T09:48:37","guid":{"rendered":"https:\/\/phd.hi.is\/doktorsvorn\/doktorsvorn-i-efnafraedi-anna-lena-johanna-segler\/"},"modified":"2026-10-19T09:48:37","modified_gmt":"2026-10-19T09:48:37","slug":"doktorsvorn-i-efnafraedi-anna-lena-johanna-segler","status":"publish","type":"phd_defence","link":"https:\/\/phd.hi.is\/en\/doktorsvorn\/doktorsvorn-i-efnafraedi-anna-lena-johanna-segler\/","title":{"rendered":"Doktorsv\u00f6rn \u00ed efnafr\u00e6\u00f0i – Anna-Lena Johanna Segler"},"content":{"rendered":"
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Streymi<\/strong><\/a><\/p>\n

Doktorsefni:<\/strong>\u00a0Anna-Lena Johanna Segler<\/p>\n

Heiti ritger\u00f0ar:<\/strong>\u00a0Efnasm\u00ed\u00f0i \u00e1 DNA og RNA sem innihalda st\u00edf spuna- og fl\u00farlj\u00f3mandi merki til ranns\u00f3kna me\u00f0 EPR- og fl\u00farlj\u00f3munarlitr\u00f3fsgreiningum (Synthesis of DNA and RNA containing rigid spin- and fluorescent labels for studies by EPR and fluorescence spectroscopies)<\/p>\n

Andm\u00e6lendur:
<\/strong>Dr. Stef\u00e1n J\u00f3nsson, framkv\u00e6mdastj\u00f3ri hj\u00e1 Alvotech
Dr. Claudia H\u00f6bartner, pr\u00f3fessor vi\u00f0 Efnafr\u00e6\u00f0i- og lyfjafr\u00e6\u00f0ideild H\u00e1sk\u00f3lans \u00ed W\u00fcrzburg, \u00de\u00fdskalandi<\/p>\n

Lei\u00f0beinandi:<\/strong>\u00a0Dr. Snorri \u00de\u00f3r Sigur\u00f0sson, pr\u00f3fessor vi\u00f0 Raunv\u00edsindadeild H\u00e1sk\u00f3la \u00cdslands<\/p>\n

Einnig \u00ed doktorsnefnd:
<\/strong>Dr. \u00d3ttar Rolfsson, pr\u00f3fessor vi\u00f0 L\u00e6knadeild H\u00e1sk\u00f3la \u00cdslands
Dr. Gu\u00f0mundur G. Haraldsson, pr\u00f3fessor vi\u00f0 Raunv\u00edsindadeild H\u00e1sk\u00f3la \u00cdslands
Dr. Benjam\u00edn Ragnar Sveinbj\u00f6rnsson, lektor vi\u00f0 Raunv\u00edsindadeild H\u00e1sk\u00f3la \u00cdslands<\/p>\n

Doktorsv\u00f6rn st\u00fdrir:<\/strong>\u00a0Dr. Einar \u00d6rn Sveinbj\u00f6rnsson, pr\u00f3fessor og deildarforseti Raunv\u00edsindadeildar H\u00e1sk\u00f3la \u00cdslands<\/p>\n

\u00c1grip<\/strong><\/p>\n

Til a\u00f0 f\u00e1 vitneskju um virkni kjarns\u00fdra er nau\u00f0synlegt a\u00f0 rannsaka byggingu og hreyfingu \u00feeirra. Litr\u00f3fsgreiningar sem byggja \u00e1 rafeindasegul\u00f3mun (e. electron paramagnetic resonance, EPR) og fl\u00farlj\u00f3mun eru mj\u00f6g n\u00e6mar og gagnlegar a\u00f0fer\u00f0ir til a\u00f0 rannsaka kjarns\u00fdrur. Til a\u00f0 unnt s\u00e9 a\u00f0 beita sl\u00edkum m\u00e6lia\u00f0fer\u00f0um er nau\u00f0synlegt a\u00f0 innlei\u00f0a me\u00f0seglandi og fl\u00farlj\u00f3mandi merki inn \u00ed kjarns\u00fdrurnar. \u00deessi doktorsritger\u00f0 l\u00fdsir efnasm\u00ed\u00f0i \u00e1 st\u00edfum spuna- og fl\u00farlj\u00f3mandi merkjum og innlei\u00f0ingu \u00feeirra inn \u00ed DNA og RNA til ranns\u00f3kna \u00e1 byggingu og hreyfingu kjarns\u00fdra me\u00f0 EPR- og fl\u00farlj\u00f3munarlitr\u00f3fsgreiningum.<\/p>\n

Fyrsti hluti ritger\u00f0arinnar l\u00fdsir hvernig teki\u00f0 var \u00e1 \u00fer\u00e1l\u00e1tu vandam\u00e1li sem tengist spunamerkingu kjarns\u00fdra, n\u00e1nar tilteki\u00f0 afoxun n\u00edtrox\u00ed\u00f0a \u00feegar kjarns\u00fdrur eru sm\u00ed\u00f0a\u00f0ar \u00e1 f\u00f6stu efni (e. solid-phase synthesis). Bens\u00f3\u00fdl h\u00f3pur var nota\u00f0ur til a\u00f0 vernda n\u00edtrox\u00ed\u00f0i\u00f0 me\u00f0 \u00fev\u00ed a\u00f0 afoxa \u00fea\u00f0 \u00ed h\u00fddrox\u00fdlam\u00edn, sem \u00ed kj\u00f6lfari\u00f0 var bens\u00f3\u00fdla\u00f0. \u00deessi a\u00f0fer\u00f0 var notu\u00f0 til a\u00f0 innlei\u00f0a st\u00edfa spunamerki\u00f0 \u00c7m \u00f3laska\u00f0 inn \u00ed RNA. \u00deessa verndunara\u00f0fer\u00f0 \u00e6tti a\u00f0 n\u00fdtast almennt til a\u00f0 innlei\u00f0a n\u00edtrox\u00ed\u00f0-spunamerki inn \u00ed kjarns\u00fdrur me\u00f0 fosf\u00f3ramid\u00ed\u00f0-efnasm\u00ed\u00f0um (e. phosphoramidite approach).<\/p>\n

\u00cd \u00f6\u00f0rum hluta ritger\u00f0arinnar er fjalla\u00f0 um merkingu \u00e1kve\u00f0inna kjarns\u00fdra me\u00f0 st\u00edfum spuna- og fl\u00farlj\u00f3mandi merkjum, n\u00e1nar tilteki\u00f0 \u00c7, \u00c7m og \u00c7mf. St\u00edfu spunamerkin \u00c7 og \u00c7m voru innleidd \u00ed DNA og RNA sameindir til ranns\u00f3kna \u00ed ranns\u00f3knarh\u00f3pi pr\u00f3fessor Thomas F. Prisner vi\u00f0 Goethe H\u00e1sk\u00f3lann \u00ed Frankfurt, me\u00f0 PELDOR (e. pulsed electron-electron double resonance) litr\u00f3fsgreiningu. \u00dear a\u00f0 auki var \u00c7mf, fl\u00farlj\u00f3mandi aflei\u00f0an af\u00a0 \u00c7m, innleidd \u00ed \u00fdmsar RNA sameindir til ranns\u00f3kna me\u00f0 fl\u00farlj\u00f3mun \u00ed ranns\u00f3knarh\u00f3pi pr\u00f3fessor Josefs Wachtveitl vi\u00f0 Goethe-h\u00e1sk\u00f3lann \u00ed Frankfurt.<\/p>\n

Loks l\u00fdsir \u00feri\u00f0ji hluti ritger\u00f0arinnar h\u00f6nnun og sm\u00ed\u00f0i \u00e1 n\u00fdjum st\u00edfum spunamerkjum, \u010a og \u010am, fyrir DNA og RNA, sem hafa karbas\u00f3lbyggingu og eru aflei\u00f0ur af s\u00fdtid\u00edni. Kristalgreining bendir til a\u00f0 \u010a og \u010am s\u00e9u enn st\u00edfari en spunamerkin \u00c7 og \u00c7m. \u010a og \u010am voru innleidd \u00ed \u00fdmis f\u00e1kirni (e. oligonucleotides) og var s\u00fdnt fram \u00e1 a\u00f0 \u010a og \u010am raska ekki byggingu tv\u00edstrendinga (e. duplex). \u00deessi n\u00fdju st\u00edfu spunamerki eru v\u00e6nleg fyrir ranns\u00f3knir \u00e1 byggingu og hreyfingu DNA og RNA me\u00f0 p\u00falsu\u00f0um EPR m\u00e6lingum.<\/p>\n

Um doktorsefni\u00f0<\/strong><\/p>\n

Anna-Lena f\u00e6ddist \u00ed Rendsburg \u00ed \u00de\u00fdskalandi \u00e1ri\u00f0 1990 en flutti \u00e1samt fj\u00f6lskyldu sinni til \u00cdslands \u00e1ri\u00f0 1998. H\u00fan lauk B.Sc. gr\u00e1\u00f0u \u00ed efnafr\u00e6\u00f0i vi\u00f0 H\u00e1sk\u00f3la \u00cdslands \u00e1ri\u00f0 2016. Anna-Lena h\u00f3f meistaran\u00e1m \u00ed efnafr\u00e6\u00f0i vi\u00f0 H\u00e1sk\u00f3la \u00cdslands \u00e1ri\u00f0 2016 en skipti yfir \u00ed doktorsn\u00e1m \u00e1ri\u00f0 2017.<\/p>\n<\/div><\/div>","protected":false},"featured_media":4966,"template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_phd_date_start":"2021-09-09T10:00:00+00:00","_phd_date_end":"2021-09-09T12:00:00+00:00","_phd_location":"Fyrirlestrarsalur \u00dej\u00f3\u00f0minjasafnsins","_phd_admission":"A\u00f0gangur \u00f3keypis","_phd_thesis_title":"Efnasm\u00ed\u00f0i \u00e1 DNA og RNA sem innihalda st\u00edf spuna- og fl\u00farlj\u00f3mandi merki til ranns\u00f3kna me\u00f0 EPR- og fl\u00farlj\u00f3munarlitr\u00f3fsgreiningum (Synthesis of DNA and RNA containing rigid spin- and fluorescent labels for studies by EPR and fluorescence spectroscopies)","_phd_source_url":"https:\/\/gamli.hi.is\/vidburdir\/doktorsvorn_i_efnafraedi_anna_lena_johanna_segler","_phd_source_node_id":"378651","_phd_source_type":"vidburdir","_phd_member_id":725,"footnotes":""},"phd_school":[101,93],"phd_discipline":[102],"class_list":["post-6202","phd_defence","type-phd_defence","status-publish","has-post-thumbnail","hentry","phd_school-raunvisindadeild","phd_school-verkfraedi-og-natturuvisindasvid","phd_discipline-phd-i-efnafraedi"],"_links":{"self":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence\/6202","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence"}],"about":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/types\/phd_defence"}],"version-history":[{"count":0,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence\/6202\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/media\/4966"}],"wp:attachment":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/media?parent=6202"}],"wp:term":[{"taxonomy":"phd_school","embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_school?post=6202"},{"taxonomy":"phd_discipline","embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_discipline?post=6202"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}