{"id":6090,"date":"2026-10-19T09:48:27","date_gmt":"2026-10-19T09:48:27","guid":{"rendered":"https:\/\/phd.hi.is\/doktorsvorn\/doktorsvorn-i-efnafraedi-nilesh-r-kamble\/"},"modified":"2026-10-19T09:48:27","modified_gmt":"2026-10-19T09:48:27","slug":"doktorsvorn-i-efnafraedi-nilesh-r-kamble","status":"publish","type":"phd_defence","link":"https:\/\/phd.hi.is\/en\/doktorsvorn\/doktorsvorn-i-efnafraedi-nilesh-r-kamble\/","title":{"rendered":"Doktorsv\u00f6rn \u00ed efnafr\u00e6\u00f0i &#8211; Nilesh R. Kamble"},"content":{"rendered":"<div class=\"field-items\">\n<div class=\"field-item even\" property=\"content:encoded\">\n<p><strong>Doktorsefni:<\/strong> Nilesh R. Kamble<\/p>\n<p><strong>Heiti ritger\u00f0ar:<\/strong> P\u00farin-afleidd n\u00edtrox\u00ed\u00f0 til spunamerkinga \u00e1n samgildra tengja \u00e1 basalausum st\u00f6\u00f0um \u00ed tv\u00ed\u00fe\u00e1tta kjarns\u00fdrum.<\/p>\n<p><strong>Andm\u00e6lendur:<\/strong><\/p>\n<p>Dr. Stef\u00e1n J\u00f3nsson, verkefnisstj\u00f3ri hj\u00e1 \u00cdslenskri erf\u00f0agreiningu,<br \/>\nDr. Janet Lovett, Royal Society University Research Fellow vi\u00f0 H\u00e1sk\u00f3lann \u00ed St Andrews, Skotlandi.<\/p>\n<p><strong>Lei\u00f0beinandi:<\/strong> Dr. Snorri \u00de\u00f3r Sigur\u00f0sson, pr\u00f3fessor vi\u00f0 Raunv\u00edsindadeild H\u00e1sk\u00f3la \u00cdslands.<\/p>\n<p><strong>A\u00f0rir \u00ed doktorsnefnd: <\/strong>Dr. Gu\u00f0mundur G. Haraldsson, pr\u00f3fessor vi\u00f0 Raunv\u00edsindadeild H\u00e1sk\u00f3la \u00cdslands, og dr. M\u00e1r M\u00e1sson, pr\u00f3fessor vi\u00f0 Lyfjafr\u00e6\u00f0ideild H\u00e1sk\u00f3la \u00cdslands.<\/p>\n<p><strong>Doktorsv\u00f6rn st\u00fdrir:<\/strong> Dr. Oddur Ing\u00f3lfsson, deildarforseti Raunv\u00edsindadeildar.<\/p>\n<p><strong>\u00c1grip af ranns\u00f3kn: <\/strong>Rafeindaspunalitr\u00f3fsgreining (e. electron paramagnetic resonance (EPR) spectroscopy) er vel \u00feekkt a\u00f0fer\u00f0 sem er reglubundi\u00f0 beitt \u00ed ranns\u00f3knum \u00e1 byggingu og hreyfingu kjarns\u00fdra. EPR m\u00e6lingar grundvallast \u00e1 \u00fev\u00ed a\u00f0 koma me\u00f0seglandi kjarna fyrir \u00e1 \u00e1kve\u00f0num sta\u00f0 innan kjarns\u00fdra, \u00fe. e. sta\u00f0bundnum spunamerkingum (e. site directed spin-labeling, SDSL). Spunamerkingara\u00f0fer\u00f0in sem h\u00e9r er l\u00fdst byggist \u00e1 v\u00edxlverkun milli tengils og vi\u00f0taka, \u00fear sem spunamerki\u00f0 binst vi\u00f0 basalausa st\u00f6\u00f0u (basaey\u00f0u) \u00ed tv\u00ed\u00fe\u00e1tta kjarns\u00fdrum, annars vegar me\u00f0 Watson-Crick basap\u00f6run vi\u00f0 staka basann \u00e1 m\u00f3tst\u00e6\u00f0a strendingnum og hins vegar me\u00f0 p\u00ed-st\u00f6flun vi\u00f0 a\u00f0liggjandi basa. Safn p\u00far\u00edn-tengdra spunamerkja var \u00fatb\u00fai\u00f0 me\u00f0 b\u00e6\u00f0i 1,1,3,3-tetramet\u00fdl\u00eds\u00f3ind\u00f3l\u00edn-2-\u00fdlox\u00fdl og 2,2,6,6-tetramet\u00fdlpiperid\u00edn-N-ox\u00fdl (TEMPO) merkih\u00f3pum, sem voru tengdir inn \u00e1 C2- e\u00f0a C6-st\u00f6\u00f0una \u00e1 p\u00far\u00edni. \u00deannig fengust n\u00edtrox\u00ed\u00f0 aflei\u00f0ur g\u00faan\u00edns, aden\u00edns e\u00f0a 2,6-d\u00edam\u00edn\u00f3p\u00far\u00edns. Tengigeta \u00feessara spunamerktu p\u00far\u00edna vi\u00f0 basaey\u00f0u \u00ed tv\u00ed\u00fe\u00e1tta kjarns\u00fdrum var m\u00e6ld me\u00f0 EPR m\u00e6lingu. Aflei\u00f0ur \u00eds\u00f3ind\u00f3l\u00edn spunamerkjanna tengdust fullkomlega vi\u00f0 sl\u00edkar ey\u00f0ur \u00ed tv\u00ed\u00fe\u00e1tta RNA og mun betur en TEMPO aflei\u00f0urnar. G\u00faan\u00edn aflei\u00f0an \u01f4 tengdist a\u00f0 fullu vi\u00f0 b\u00e6\u00f0i tv\u00ed\u00fe\u00e1tta DNA og RNA vi\u00f0 l\u00e1gt hitastig, \u00feegar h\u00fan var p\u00f6ru\u00f0 vi\u00f0 s\u00fdt\u00f3s\u00edn (C). \u01f4 tengdist betur vi\u00f0 RNA en DNA og s\u00fdndi jafnvel mikla bindingu vi\u00f0 20 \u00b0C (Kd = 6,15\u221910-6 M). Aden\u00edn-afleidda spunamerki\u00f0 5 s\u00fdndi einnig h\u00e1marks tengingu vi\u00f0 l\u00e1gt hitastig (-30 \u00b0C) vi\u00f0 basaey\u00f0una \u00e1 tv\u00ed\u00fe\u00e1tta DNA og RNA \u00e1 m\u00f3ti T annars vegar og U hins vegar. Spunamerki\u00f0 \u01f4 var s\u00ed\u00f0an vali\u00f0 til a\u00f0 rannsaka n\u00e1nar \u00fdmsa \u00fe\u00e6tti spunamerkingar \u00e1n samgildra tengja. \u00cd lj\u00f3s kom a\u00f0 \u01f4 tengdist basaey\u00f0um \u00ed DNA\/RNA blendingum, anna\u00f0hvort vi\u00f0 DNA- e\u00f0a RNA strendinginn. Basarnir vi\u00f0 hli\u00f0ina \u00e1 basalausu st\u00f6\u00f0unni h\u00f6f\u00f0u a\u00f0eins l\u00edtil \u00e1hrif \u00e1 tengingu \u01f4 vi\u00f0 ey\u00f0una \u00ed RNA. \u00deegar sl\u00edk ey\u00f0a var sta\u00f0sett n\u00e1l\u00e6gt endandum \u00e1 tv\u00ed\u00fe\u00e1tta kjarns\u00fdrunni, minnka\u00f0i s\u00e6kni spunamerkisins, v\u00e6ntanlega vegna aukins hreyfanleika basapara sem sta\u00f0sett eru \u00ed n\u00e1nd vi\u00f0 endann. Fjarl\u00e6g\u00f0am\u00e6ling milli tveggja spunamerkja var framkv\u00e6md \u00e1 \u01f4-merktu tv\u00ed\u00fe\u00e1tta RNA me\u00f0 notkun PELDOR (e. pulsed electron-electron double resonance) \u00ed samvinnu vi\u00f0 Prof. Thomas Prisner vi\u00f0 Goethe H\u00e1sk\u00f3lann \u00ed Frankfurt, \u00de\u00fdskalandi. PELDOR m\u00e6lingarnar voru h\u00e1\u00f0ar innbyr\u00f0is afst\u00f6\u00f0u (e. orientation dependence) spunamerkjanna og g\u00e1fu fjarl\u00e6g\u00f0ina 31,3 \u00b1 3,5 \u00c5, sem reyndist \u00ed g\u00f3\u00f0u samr\u00e6mi vi\u00f0 fjarl\u00e6g\u00f0ina \u00ed l\u00edk\u00f6num (29 \u00c5).<\/p>\n<p><strong>Um doktorsefni\u00f0:<\/strong> Nilesh Kamble f\u00e6ddist \u00e1ri\u00f0 1980 \u00ed Pune \u00ed Maharashtra r\u00edki \u00e1 Indlandi. Hann \u00fatskrifa\u00f0ist me\u00f0 B.Sc.-gr\u00e1\u00f0u \u00ed efnafr\u00e6\u00f0i fr\u00e1 H\u00e1sk\u00f3lanum \u00ed Pune \u00e1 Indlandi \u00e1ri\u00f0 2002 og M.Sc.-gr\u00e1\u00f0u \u00ed l\u00edfr\u00e6nni efnafr\u00e6\u00f0i fr\u00e1 sama h\u00e1sk\u00f3la \u00e1ri\u00f0 2004. Eftir a\u00f0 hafa unni\u00f0 fyrir lyfjafyrirt\u00e6ki \u00ed r\u00fam n\u00edu \u00e1r h\u00f3f hann doktorsn\u00e1m vi\u00f0 H\u00e1sk\u00f3la \u00cdslands. Nilesh er giftur Bhakti Kamble og eiga \u00feau einn son, Mayank.<\/p>\n<p><a href=\"https:\/\/www.facebook.com\/events\/556765974665620\/\">Facebook vi\u00f0bur\u00f0ur<\/a><\/p>\n<\/div><\/div>","protected":false},"featured_media":1840,"template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_phd_date_start":"2017-12-21T10:00:00+00:00","_phd_date_end":"2017-12-21T13:00:00+00:00","_phd_location":"Askja N-132","_phd_admission":"A\u00f0gangur \u00f3keypis","_phd_thesis_title":"P\u00farin-afleidd n\u00edtrox\u00ed\u00f0 til spunamerkinga \u00e1n samgildra tengja \u00e1 basalausum st\u00f6\u00f0um \u00ed tv\u00ed\u00fe\u00e1tta kjarns\u00fdrum","_phd_source_url":"https:\/\/gamli.hi.is\/vidburdir\/doktorsvorn_i_efnafraedi_nilesh_r_kamble","_phd_source_node_id":"309274","_phd_source_type":"vidburdir","_phd_member_id":476,"footnotes":""},"phd_school":[101,93],"phd_discipline":[102],"class_list":["post-6090","phd_defence","type-phd_defence","status-publish","has-post-thumbnail","hentry","phd_school-raunvisindadeild","phd_school-verkfraedi-og-natturuvisindasvid","phd_discipline-phd-i-efnafraedi"],"_links":{"self":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence\/6090","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence"}],"about":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/types\/phd_defence"}],"version-history":[{"count":0,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_defence\/6090\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/media\/1840"}],"wp:attachment":[{"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/media?parent=6090"}],"wp:term":[{"taxonomy":"phd_school","embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_school?post=6090"},{"taxonomy":"phd_discipline","embeddable":true,"href":"https:\/\/phd.hi.is\/en\/wp-json\/wp\/v2\/phd_discipline?post=6090"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}